pCAMBIA1303-TrpC-Hygro-gpdA-GFP

LOCUS       pCAMBIA1303-TrpC-Hygro-gpdA-GFP      12765 bp ds-DNA     circular SYN 22-SEP-2016
DEFINITION  synthetic circular DNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12765)
  AUTHORS   .
  TITLE     Direct Submission
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..12765
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             14..1819
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA
                     VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM
                     EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF
                     FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV
                     VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQFL
                     INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA
                     DEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
                     NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             1823..2533
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             2540..2557
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2589..2841
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2863..2887
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4187..4816
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5250..6317
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6383..6577
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6921..7061
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7247..7835)
                     /direction=LEFT
                     /label=pUC ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7922..8716)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9141..9165
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    9243..9417
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    9424..9654
                     /label=TrpC terminator
     CDS             complement(9740..10765)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     misc_feature    complement(10766..11151)
                     /label=TrpC promoter
     promoter        complement(11242..11343)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     misc_feature    11356..12765
                     /label=gpdA
ORIGIN
        1 agatctgact agtttacgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg
       61 cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag
      121 cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga
      181 tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa
      241 aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt
      301 gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga
      361 tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga
      421 actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa
      481 gcagtcttac ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta
      541 caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg
      601 taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg
      661 tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt
      721 gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa
      781 aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa
      841 gggccaacag ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga
      901 agatgcggac ttacgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt
      961 aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat
     1021 gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt
     1081 tcagctgtct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga
     1141 agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc
     1201 gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg
     1261 tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc
     1321 gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag
     1381 cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc aaagcggcga
     1441 tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg agaaactgca
     1501 tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta
     1561 caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt
     1621 tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc
     1681 gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc
     1741 gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc
     1801 gcagcaggga ggcaaacaag ctagtaaagg agaagaactt ttcactggag ttgtcccaat
     1861 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
     1921 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
     1981 tgttccgtgg ccaacacttg tcactacttt ctcttatggt gttcaatgct tttcaagata
     2041 cccagatcat atgaagcggc acgacttctt caagagcgcc atgcctgagg gatacgtgca
     2101 ggagaggacc atcttcttca aggacgacgg gaactacaag acacgtgctg aagtcaagtt
     2161 tgagggagac accctcgtca acaggatcga gcttaaggga atcgatttca aggaggacgg
     2221 aaacatcctc ggccacaagt tggaatacaa ctacaactcc cacaacgtat acatcatggc
     2281 cgacaagcaa aagaacggca tcaaagccaa cttcaagacc cgccacaaca tcgaagacgg
     2341 cggcgtgcaa ctcgctgatc attatcaaca aaatactcca attggcgatg gccctgtcct
     2401 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
     2461 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     2521 tgaactatac aaagctagcc accaccacca ccaccacgtg tgaattggtg accagctcga
     2581 atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     2641 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     2701 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     2761 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     2821 tgtcatctat gttactagat cgggaattaa actatcagtg tttgacagga tatattggcg
     2881 ggtaaaccta agagaaaaga gcgtttatta gaataacgga tatttaaaag ggcgtgaaaa
     2941 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa
     3001 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
     3061 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
     3121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
     3181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
     3241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
     3301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
     3361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
     3421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
     3481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
     3541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
     3601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
     3661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
     3721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
     3781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
     3841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
     3901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
     3961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     4021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     4081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     4141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     4201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     4261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     4321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     4381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     4441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     4501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     4561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     4621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     4681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     4741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     4801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     4861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     4921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     4981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     5041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     5101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     5161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     5221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
     5281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     5341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     5401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     5461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     5521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     5581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     5641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     5701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     5761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     5821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     5881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     5941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     6001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     6061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     6121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     6181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     6241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     6301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     6361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     6421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     6481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     6541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     6601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     6661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     6721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     6781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     6841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     6901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     6961 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     7021 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     7081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt
     7921 actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc
     7981 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc
     8041 ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa
     8101 agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa
     8161 agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt
     8221 taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta
     8281 agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt
     8341 cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt
     8401 gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc
     8461 tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc
     8521 atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg
     8581 tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca
     8641 ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata
     8701 ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca
     8761 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa
     8821 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
     8881 ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca
     8941 acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt
     9001 ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg
     9061 ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
     9121 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga
     9181 caacttaata acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcgg
     9241 gggatctgga ttttagtact ggattttggt tttaggaatt agaaatttta ttgatagaag
     9301 tattttacaa atacaaatac atactaaggg tttcttatat gctcaacaca tgagcgaaac
     9361 cctataggaa ccctaattcc cttatctggg aactactcac acattattat ggagaaactc
     9421 gaggcattgc agatgagctg tatctggaag aggtaaaccc gaaacgcgtt ttattcttgt
     9481 tgacatggag ctattaaatc actagaaggc actctttgct gcttggacaa atgaacgtat
     9541 cttatcgaga tcctgaacac catttgtctc aactccggag ctgacatcga caccaacgat
     9601 cttatatcca gattcgtcaa gctgtttgat gatttcagta acgttaagtg gatcatgata
     9661 atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa
     9721 cgatctgcag gtcgacggac tatttctttg ccctcggacg agtgctgggg cgtcggtttc
     9781 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga
     9841 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg
     9901 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa
     9961 tgcggagcat atacgcccgg agtcgtggcg atcctgcaag ctccggatgc ctccgctcga
    10021 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga
    10081 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc
    10141 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg
    10201 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg
    10261 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat
    10321 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct
    10381 ggctaagatc ggccgcagcg atcgcatcca tagcctccgc gaccggttgt agaacagcgg
    10441 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat
    10501 aggtcaggct ctcgctaaac tccccaatgt caagcacttc cggaatcggg agcgcggccg
    10561 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc
    10621 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg
    10681 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg
    10741 tcgcggtgag ttcaggcttt ttcatggtta cttcctaatc gaagctttgc ttgggtagaa
    10801 taggtaagtc agattgaatc tgaaataaag ggaggaaggg cgaacttaag aaggtatgac
    10861 cgggtcgttc acttaccttg cttgacaaac gcaccaagtt atcgtgcacc aagcagcaga
    10921 tgataataat gtcctcgttc ctgtctgcta ataagagtca cacttcgagc gccgccgcta
    10981 ctgctacaag tggggctgat ctgaccagtt gcctaaatga accatcttgt caaacgacac
    11041 aaattttgtg ctcaccgcct ggacgactaa accaaaatag gcattcattg ttgacctcca
    11101 ctagctccag ccaagcccaa aaagtgctcc ttcaatatca tcttctgtcg agtctagagc
    11161 acattctgcg gcctctgtgc tctgcaagcc gcaaactttc accaatggac cagaactacc
    11221 tgtgaaatta ataacagaca tactccaagc tgcctttgtg tgcttaatca cgtatactca
    11281 cgtgctcaat agtcaccaat gccctccctc ttggccctct ccttttcttt tttcgaccga
    11341 attgagctcg gtaccaaacg ttgctagcag ttccaggtgg aatgttatga tgagcattgt
    11401 attaaatcag gagatatagc atgatctcta gttagctcac cacaaaagtc agacggcgta
    11461 accaaaagtc acacaacaca agctgtaagg atttcggcac ggctacggaa gacggagaag
    11521 ccaccttcag tggactcgag taccatttaa ttctatttgt gtttgatcga gacctaatac
    11581 agcccctaca acgaccatca aagtcgtata gctaccagtg aggaagtgga ctcaaatcga
    11641 cttcagcaac atctcctgga taaactttaa gcctaaacta tacagaataa gataggtgga
    11701 gagcttatac cgagctccca aatctgtcca gatcatggtt gaccggtgcc tggatcttcc
    11761 tatagaatca tccttattcg ttgacctagc tgattctgga gtgacccaga gggtcatgac
    11821 ttgagcctaa aatccgccgc ctccaccatt tgtagaaaaa tgtgacgaac tcgtgagctc
    11881 tgtacagtga ccggtgactc tttctggcat gcggagagac ggacggacgc agagagaagg
    11941 gctgagtaat aagccactgg ccagacagct ctggcggctc tgaggtgcag tggatgatta
    12001 ttaatccggg accggccgcc cctccgcccc gaagtggaaa ggctggtgtg cccctcgttg
    12061 accaagaatc tattgcatca tcggagaata tggagcttca tcgaatcacc ggcagtaagc
    12121 gaaggagaat gtgaagccag gggtgtatag ccgtcggcga aatagcatgc cattaaccta
    12181 ggtacagaag tccaattgct tccgatctgg taaaagattc acgagatagt accttctccg
    12241 aagtaggtag agcgagtacc cggcgcgtaa gctccctaat tggcccatcc ggcatctgta
    12301 gggcgtccaa atatcgtgcc tctcctgctt tgcccggtgt atgaaaccgg aaaggccgct
    12361 caggagctgg ccagcggcgc agaccgggaa cacaagctgg cagtcgaccc atccggtgct
    12421 ctgcactcga cctgctgagg tccctcagtc cctggtaggc agctttgccc cgtctgtccg
    12481 cccggtgtgt cggcggggtt gacaaggtcg ttgcgtcagt ccaacatttg ttgccatatt
    12541 ttcctgctct ccccaccagc tgctcttttc ttttctcttt cttttcccat cttcagtata
    12601 ttcatcttcc catccaagaa cctttatttc ccctaagtaa gtactttgct acatccatac
    12661 tccatccttc ccatccctta ttcctttgaa cctttcagtt cgagctttcc cacttcatcg
    12721 cagcttgact aacagctacc ccgcttgagc agacatcacc atggt
//